Anti-PorA protein [MN14C11.6]

Absolute Antibody

Anti-PorA protein [MN14C11.6]

9

This antibody recognizes the P1.7 loop of the PorA protein of Neisseria meningitidis. Epitope mapping revealed that this antibody specifically binds a sequence ASGQ. This antibody can bind both A and B serotype of Neisseria meningitidis. The major outer m

This antibody showed both bactericidal and opsonophagocytic for P1.7-expressing meningococci. The lowest concentration resulting in >50% killing of strain 44/76-SL was reported to be 0.5 microg/l. The opsonic activity of this antibody was measured against strain 44/76-SL with human peripheral blood polymorphonuclear leukocytes (PMN) as the effector cells and human serum as the complement source. This antibody demonstrated high opsonophagocytic activity with >80% of PMNs exhibiting measurable RB at a concentration of the MAbs of <10 microg/ml. This antibody was opsonic for the test strain 44/76-SL at a concentration of 0.2 microg/ml (PMID: 10722576). This antibody is not capable of binding meningococcal isolates expressing some variations of the P1.7 family especially P1.7b in a whole cell ELISA (PMID: 10512717). This antibody can be used in the detection of outer membrane protein of N. meningitidis strains like 44/76 using an ELISA (PMID: 12753502). This antibody was also used for the analysis of isogenic target strains derived from H44/76 (B:15:P1.7,16) in a western blot assay (PMID: 8698504).

Functional Assay, Western Blot, ELISA

Research Use Only

Monoclonal

MN14C11.6

Rabbit

IgG

Major outer membrane protein P.IA

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.

Bacteria

-20°C,2°C to 8°C

12352203