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Immunofluorescence staining of A-431 cells using the Anti-POU5F1 monoclonal antibody, showing specific staining in the nucleoplasm and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunofluorescence staining of A-431 cells using the Anti-POU5F1 monoclonal antibody, showing specific staining in the nucleoplasm and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunofluorescence staining of A-431 cells using the Anti-POU5F1 monoclonal antibody, showing specific staining in the nucleoplasm and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Anti-POU5F1 Antibody

AMAB91477
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetPOU5F1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-POU5F1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL7621
  • Conjugate
    Unconjugated
  • Gene ID5460
  • Target name
    POU5F1
  • Target description
    POU class 5 homeobox 1
  • Target synonyms
    OCT3; Oct-3; OCT4; Oct-4; octamer-binding protein 3; octamer-binding protein 4; octamer-binding transcription factor 3; OTF3; OTF-3; OTF4; POU domain transcription factor OCT4; POU domain, class 5, transcription factor 1; POU-type homeodomain-containing DNA-binding protein
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ01860
  • Protein Name
    POU domain, class 5, transcription factor 1
  • Scientific Description
    Synthetic Peptide
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of POU5F1 using anti-POU5F1 antibody (A00174-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POU5F1 antigen affinity purified polyclonal antibody (Catalog # A00174-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POU5F1 at approximately 45 kDa. The expected band size for POU5F1 is at 39 kDa.
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