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Immunohistochemical staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.

Anti-PPA2 Antibody

HPA031671
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPPA2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PPA2 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID27068
  • Target name
    PPA2
  • Target description
    inorganic pyrophosphatase 2
  • Target synonyms
    HSPC124, SCFAI, SCFI, SID6-306, inorganic pyrophosphatase 2, mitochondrial, PPase 2, pyrophosphatase (inorganic) 2, pyrophosphatase SID6-306, pyrophosphate phospho-hydrolase 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H2U2
  • Protein Name
    Inorganic pyrophosphatase 2, mitochondrial
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of PPA2 using anti-PPA2 antibody (A06587). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human U87 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPA2 antigen affinity purified polyclonal antibody (Catalog # A06587) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPA2 at approximately 35 kDa. The expected band size for PPA2 is at 38 kDa.
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