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Immunohistochemical staining of human cerebellum shows strong cytoplasmic and nuclear positivity in Purkinje cellsm cells in molecular layer and granular layer.
Immunohistochemical staining of human cerebellum shows strong cytoplasmic and nuclear positivity in Purkinje cellsm cells in molecular layer and granular layer.
Immunohistochemical staining of human cerebellum shows strong cytoplasmic and nuclear positivity in Purkinje cellsm cells in molecular layer and granular layer.

Anti-PPM1E Antibody

HPA019263
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPPM1E
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PPM1E Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID22843
  • Target name
    PPM1E
  • Target description
    protein phosphatase, Mg2+/Mn2+ dependent 1E
  • Target synonyms
    CAMKPN, CaMKP-N, POPX1, PP2CH, caMKN, protein phosphatase 1E, ca(2+)/calmodulin-dependent protein kinase phosphatase N, caMKP-nucleus, nuclear calmodulin-dependent protein kinase phosphatase, partner of PIX 1, partner of PIX-alpha, partner of PIXA, protein phosphatase 1E (PP2C domain containing)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WY54
  • Protein Name
    Protein phosphatase 1E
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of PPM1E using anti-PPM1E antibody (A08794). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human U2OS whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPM1E antigen affinity purified polyclonal antibody (Catalog # A08794) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPM1E at approximately 84KD. The expected band size for PPM1E is at 84KD.
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