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Immunofluorescent staining of human cell line SiHa shows localization to the Golgi apparatus.
Immunofluorescent staining of human cell line SiHa shows localization to the Golgi apparatus.
Immunofluorescent staining of human cell line SiHa shows localization to the Golgi apparatus.

Anti-PPP1R15B Antibody

HPA061088
Atlas Antibodies
ApplicationsImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetPPP1R15B
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PPP1R15B
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID84919
  • Target name
    PPP1R15B
  • Target description
    protein phosphatase 1 regulatory subunit 15B
  • Target synonyms
    CREP; MSSGM2; protein phosphatase 1 regulatory subunit 15B; protein phosphatase 1, regulatory (inhibitor) subunit 15B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5SWA1
  • Protein Name
    Protein phosphatase 1 regulatory subunit 15B
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PPP1R15B using anti-PPP1R15B antibody (A10225-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP1R15B antigen affinity purified polyclonal antibody (Catalog # A10225-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP1R15B at approximately 110 kDa. The expected band size for PPP1R15B is at 79,100-110 kDa.
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