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Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.

Anti-PPP1R16A Antibody

HPA023755
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPPP1R16A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PPP1R16A Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID84988
  • Target name
    PPP1R16A
  • Target description
    protein phosphatase 1 regulatory subunit 16A
  • Target synonyms
    MYPT3, protein phosphatase 1 regulatory subunit 16A, myosin phosphatase-targeting subunit 3, protein phosphatase 1, regulatory (inhibitor) subunit 16A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96I34
  • Protein Name
    Protein phosphatase 1 regulatory subunit 16A
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PPP1R16A using anti-PPP1R16A antibody (A14650-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP1R16A antigen affinity purified polyclonal antibody (Catalog # A14650-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP1R16A at approximately 69 kDa. The expected band size for PPP1R16A is at 58 kDa.
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