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Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.

Anti-PPP1R3B Antibody

HPA028731
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPPP1R3B
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PPP1R3B Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79660
  • Target name
    PPP1R3B
  • Target description
    protein phosphatase 1 regulatory subunit 3B
  • Target synonyms
    GL, PPP1R4, PTG, protein phosphatase 1 regulatory subunit 3B, PP1 subunit R4, hepatic glycogen-targeting protein phosphatase 1 regulatory subunit GL, hepatic glycogen-targeting subunit, G(L), protein phosphatase 1 regulatory subunit 4, protein phosphatase 1, regulatory (inhibitor) subunit 3B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ86XI6
  • Protein Name
    Protein phosphatase 1 regulatory subunit 3B
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of PPP1R3B using anti-PPP1R3B antibody (A09697). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP1R3B antigen affinity purified polyclonal antibody (Catalog # A09697) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP1R3B at approximately 33 kDa. The expected band size for PPP1R3B is at 33 kDa.
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