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Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells and in neuropil.
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells and in neuropil.
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells and in neuropil.

Anti-PPP1R9A Antibody

HPA027726
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPPP1R9A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PPP1R9A Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55607
  • Target name
    PPP1R9A
  • Target description
    protein phosphatase 1 regulatory subunit 9A
  • Target synonyms
    NRB1, NRBI, Neurabin-I, neurabin-1, neural tissue-specific F-actin-binding protein I, protein phosphatase 1, regulatory (inhibitor) subunit 9A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9ULJ8
  • Protein Name
    Neurabin-1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PPP1R9A using anti-PPP1R9A antibody (A07331-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP1R9A antigen affinity purified polyclonal antibody (Catalog # A07331-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP1R9A at approximately 190 kDa. The expected band size for PPP1R9A is at 123 kDa.
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