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Immunohistochemical staining of human hippocampus shows moderate cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human hippocampus shows moderate cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human hippocampus shows moderate cytoplasmic positivity in neuronal cells.

Anti-PPP2R3A Antibody

HPA035829
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPPP2R3A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PPP2R3A Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5523
  • Target name
    PPP2R3A
  • Target description
    protein phosphatase 2 regulatory subunit B''alpha
  • Target synonyms
    PPP2R3, PR130, PR72, serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit alpha, PR130/B''alpha1 subunit, PR72/B''alpha2 subuit, protein phosphatase 2, regulatory subunit B'', alpha, serine/threonine-protein phosphatase 2A 72/130 kDa regulatory subunit B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ06190
  • Protein Name
    Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit alpha
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PPP2R3A using anti-PPP2R3A antibody (A11620-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP2R3A antigen affinity purified polyclonal antibody (Catalog # A11620-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP2R3A at approximately 128 kDa. The expected band size for PPP2R3A is at 48,61,130 kDa.
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