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Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.
Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.
Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.

Anti-PPP2R5A Antibody

HPA078841
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPPP2R5A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PPP2R5A
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5525
  • Target name
    PPP2R5A
  • Target description
    protein phosphatase 2 regulatory subunit B'alpha
  • Target synonyms
    B56A; B56alpha; PR61A; PR61alpha; protein phosphatase 2 regulatory subunit B', alpha; serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15172
  • Protein Name
    Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PPP2R5A using anti-PPP2R5A antibody (A05935-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP2R5A antigen affinity purified polyclonal antibody (Catalog # A05935-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP2R5A at approximately 56 kDa. The expected band size for PPP2R5A is at 56 kDa.
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