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Immunohistochemical staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells.

Anti-PPP3R1 Antibody

HPA047157
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetPPP3R1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PPP3R1
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5534
  • Target name
    PPP3R1
  • Target description
    protein phosphatase 3 regulatory subunit B, alpha
  • Target synonyms
    calcineurin B, type I (19kDa); calcineurin subunit B type 1; CALNB1; CNB; CNB1; protein phosphatase 2B regulatory subunit 1; protein phosphatase 2B regulatory subunit B alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP63098
  • Protein Name
    Calcineurin subunit B type 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Western blot All lanes: PPP3R1 antibody at 1microg/ml Lane 1: K562 whole cell lysate Lane 2: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 20 kDa Observed band size: 20 kDa
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Figure 1. Western blot analysis of PPP3R1 using anti-PPP3R1 antibody (A06469-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP3R1 antigen affinity purified polyclonal antibody (Catalog # A06469-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP3R1 at approximately 15 kDa. The expected band size for PPP3R1 is at 15,19 kDa.
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