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Immunohistochemical staining of human bone marrow shows moderate cytoplasmic positivity in hematopoietic cells.
Immunohistochemical staining of human bone marrow shows moderate cytoplasmic positivity in hematopoietic cells.
Immunohistochemical staining of human bone marrow shows moderate cytoplasmic positivity in hematopoietic cells.

Anti-PRAM1 Antibody

HPA050161
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPRAM1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PRAM1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID84106
  • Target name
    PRAM1
  • Target description
    PML-RARA regulated adaptor molecule 1
  • Target synonyms
    PML-RAR, PRAM-1, PML-RARA-regulated adapter molecule 1, PML-RARA target gene encoding an Adaptor Molecule-1, PRAM
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96QH2
  • Protein Name
    PML-RARA-regulated adapter molecule 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of PRAM1 using anti-PRAM1 antibody (A10037-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRAM1 antigen affinity purified polyclonal antibody (Catalog # A10037-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRAM1 at approximately 90 kDa. The expected band size for PRAM1 is at 70,74,90-100 kDa.
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Immunofluorescent staining of human cell line NB-4 shows localization to the Golgi apparatus.
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