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Immunofluorescence staining of U-2 OS cells using the Anti-PRAME monoclonal antibody, showing specific staining in the nucleus and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunofluorescence staining of U-2 OS cells using the Anti-PRAME monoclonal antibody, showing specific staining in the nucleus and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunofluorescence staining of U-2 OS cells using the Anti-PRAME monoclonal antibody, showing specific staining in the nucleus and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Anti-PRAME Antibody

AMAB91329
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetPRAME
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PRAME
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL5146
  • Conjugate
    Unconjugated
  • Gene ID23532
  • Target name
    PRAME
  • Target description
    PRAME nuclear receptor transcriptional regulator
  • Target synonyms
    cancer/testis antigen 130; CT130; MAPE; melanoma antigen preferentially expressed in tumors; OIP4; OIP-4; opa-interacting protein 4; Opa-interacting protein OIP4; preferentially expressed antigen in melanoma; preferentially expressed antigen of melanoma
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP78395
  • Protein Name
    Melanoma antigen preferentially expressed in tumors
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PRAME using anti-PRAME antibody (A06628-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRAME antigen affinity purified polyclonal antibody (Catalog # A06628-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRAME at approximately 58KD. The expected band size for PRAME is at 58KD.
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