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ProductsBack/Anti-PRD Rabbit Monoclonal Antibody
Western blot analysis of PRD expression in HepG2 cell lysate.
Western blot analysis of PRD expression in HepG2 cell lysate.
Western blot analysis of PRD expression in HepG2 cell lysate.

Anti-PRD Rabbit Monoclonal Antibody

M03417-2
Boster Bio
ApplicationsWestern Blot
Product group Antibodies
TargetPEPD
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-PRD Rabbit Monoclonal Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    30P28
  • Gene ID5184
  • Target name
    PEPD
  • Target description
    peptidase D
  • Target synonyms
    PROLIDASE, xaa-Pro dipeptidase, X-Pro dipeptidase, aminoacyl-L-proline hydrolase, imidodipeptidase, proline dipeptidase, testicular tissue protein Li 138
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP12955
  • Protein Name
    Xaa-Pro dipeptidase
  • Scientific Description
    Boster Bio Anti-PRD Rabbit Monoclonal Antibody catalog # M03417-2. Tested in WB application. This antibody reacts with Human.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PEPD using anti-PEPD antibody (A03417-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat small intestine tissue lysates, Lane 6: mouse small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PEPD antigen affinity purified polyclonal antibody (Catalog # A03417-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PEPD at approximately 60 kDa. The expected band size for PEPD is at 55 kDa.
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