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Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity.
Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity.
Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity.

Anti-PRDM15 Antibody

HPA024268
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPRDM15
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PRDM15 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID63977
  • Target name
    PRDM15
  • Target description
    PR/SET domain 15
  • Target synonyms
    C21orf83, PFM15, ZNF298, PR domain zinc finger protein 15, PR domain 15, PR domain containing 15, zinc finger protein 298
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP57071
  • Protein Name
    PR domain zinc finger protein 15
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of PRDM15 using anti-PRDM15 antibody (A13548-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRDM15 antigen affinity purified polyclonal antibody (Catalog # A13548-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRDM15 at approximately 169 kDa. The expected band size for PRDM15 is at 168,62 kDa.
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