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Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in acrosomes.
Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in acrosomes.
Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in acrosomes.

Anti-PRDM7 Antibody

HPA059944
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPRDM7
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PRDM7
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11105
  • Target name
    PRDM7
  • Target description
    PR/SET domain 7
  • Target synonyms
    PFM4, ZNF910, histone-lysine N-methyltransferase PRDM7, PR domain 7, PR domain containing 7, PR domain zinc finger protein 7, PR-domain family protein 4, [histone H3]-lysine4 N-methyltransferase PRDM7, probable histone-lysine N-methyltransferase PRDM7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NQW5
  • Protein Name
    Probable histone-lysine N-methyltransferase PRDM7
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of PRDM7 using anti-PRDM7 antibody (A13923-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRDM7 antigen affinity purified polyclonal antibody (Catalog # A13923-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRDM7 at approximately 46 kDa. The expected band size for PRDM7 is at 56 kDa.
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