Anti-pre-fusion viral F protein [DS7]

Absolute Antibody

Anti-pre-fusion viral F protein [DS7]

9

The binding characterization of this antibody to hMPV F protein was done using ELISA. The binding of the antibody was also confirmed using immunofluorescent assays of LLC-MK2 cell culture monolayers that were infected with hMPV. The fab version of this antibody neutralized the parent strain of hMPV with a 60% plaque reduction activity of 1.1 microg/ml and bound to hMPV F with an affinity of 9.8 x 10-10 M. This antibody was reported to reduce viral titers in the lungs of cotton rats when given therapeutically and modestly reduced titers in nasal tissues. There was a dose-response relationship between the dose of this antibody clone and viral titers. This antibody was capable of neutralizing strains from the A1 and B1 lineages in addition to the A2 lineage but failed to neutralize the B2 strain (Williams et al., 2007; PMID: 17522220). This antibody was shown to bind both pre- and post-fusion conformations of the hMPV F protein as tested via EM. Crystallography shows that the Fab version of the antibody forms a complex with a specific ~45 kD N-terminal fragment of the F protein. This fragment includes DI, DII, and parts of DIII, with the antibody binding mainly to DI (through heavy chain interactions) and the DI-DII interface (light chain interactions). In slight contradiction to previous reports, this antibody successfully neutralized representative strains of all hMPV genotypes, including the B2 lineage (Wen et al., 2012; PMID: 22388735) (Huang et al., 2019; PMID: 31849961).

Electron Microscopy, ImmunoFluorescence, ELISA, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

DS7

F

fusion glycoprotein precursor

HmVgp4, fusion glycoprotein precursor

Human

IgG1

Fusion glycoprotein F0

Virus

-20°C,2°C to 8°C

41116161