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Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.

Anti-PREX1 Antibody

HPA001927
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPREX1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PREX1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57580
  • Target name
    PREX1
  • Target description
    phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 1
  • Target synonyms
    phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein; PIP3 dependent Rac exchange factor 1; P-REX1; ptdIns(3,4,5)-dependent Rac exchanger 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TCU6
  • Protein Name
    Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of PREX1 using anti-PREX1 antibody (A03098-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PREX1 antigen affinity purified polyclonal antibody (Catalog # A03098-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PREX1 at approximately 186 kDa. The expected band size for PREX1 is at 186 kDa.
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