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Immunohistochemistry analysis in human kidney and skeletal muscle tissues using HPA004247 antibody. Corresponding PRKAB1 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human kidney and skeletal muscle tissues using HPA004247 antibody. Corresponding PRKAB1 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human kidney and skeletal muscle tissues using HPA004247 antibody. Corresponding PRKAB1 RNA-seq data are presented for the same tissues.

Anti-PRKAB1 Antibody

HPA004247
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPRKAB1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PRKAB1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5564
  • Target name
    PRKAB1
  • Target description
    protein kinase AMP-activated non-catalytic subunit beta 1
  • Target synonyms
    5'-AMP-activated protein kinase beta-1 subunit; 5'-AMP-activated protein kinase subunit beta-1; AMP-activated protein kinase beta subunit; AMPK; AMPK beta 1; AMPK beta -1 chain; AMPK subunit beta-1; AMPKb; HAMPKb; protein kinase, AMP-activated, beta 1 non-catalytic subunit; protein kinase, AMP-activated, noncatalytic, beta-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y478
  • Protein Name
    5'-AMP-activated protein kinase subunit beta-1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of AMPK beta 1 using anti-AMPK beta 1 antibody (PB9738). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: JURKAT Whole Cell Lysate, Lane 2: PANC Whole Cell Lysate, Lane 3: K562 Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMPK beta 1 antigen affinity purified polyclonal antibody (Catalog # PB9738) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMPK beta 1 at approximately 38 kDa. The expected band size for AMPK beta 1 is at 30 kDa.
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