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Immunohistochemical staining of human prostate shows moderate to strong positivity in nuclear membrane in glandular cells.
Immunohistochemical staining of human prostate shows moderate to strong positivity in nuclear membrane in glandular cells.
Immunohistochemical staining of human prostate shows moderate to strong positivity in nuclear membrane in glandular cells.

Anti-PRKAG2 Antibody

HPA004246
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPRKAG2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PRKAG2 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51422
  • Target name
    PRKAG2
  • Target description
    protein kinase AMP-activated non-catalytic subunit gamma 2
  • Target synonyms
    AAKG, AAKG2, CMH6, H91620p, WPWS, 5'-AMP-activated protein kinase subunit gamma-2, AMPK subunit gamma-2, epididymis secretory sperm binding protein, protein kinase, AMP-activated, gamma 2 non-catalytic subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UGJ0
  • Protein Name
    5'-AMP-activated protein kinase subunit gamma-2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of PRKAG2 using anti-PRKAG2 antibody (A02758-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human placenta tissue lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKAG2 antigen affinity purified polyclonal antibody (Catalog # A02758-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRKAG2 at approximately 63 kDa. The expected band size for PRKAG2 is at 63 kDa.
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