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Immunohistochemical staining of human testis shows moderate cytoplasmic and nuclear positivity in cells in seminiferous ducts and Leydig cells.
Immunohistochemical staining of human testis shows moderate cytoplasmic and nuclear positivity in cells in seminiferous ducts and Leydig cells.
Immunohistochemical staining of human testis shows moderate cytoplasmic and nuclear positivity in cells in seminiferous ducts and Leydig cells.

Anti-PRMT5 Antibody

HPA064708
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPRMT5
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PRMT5 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10419
  • Target name
    PRMT5
  • Target description
    protein arginine methyltransferase 5
  • Target synonyms
    HRMT1L5, HSL7, IBP72, JBP1, SKB1, SKB1Hs, protein arginine N-methyltransferase 5, 72 kDa ICln-binding protein, HMT1 hnRNP methyltransferase-like 5, SKB1 homolog, histone-arginine N-methyltransferase PRMT5, jak-binding protein 1, shk1 kinase-binding protein 1 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14744
  • Protein Name
    Protein arginine N-methyltransferase 5
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of PRMT5 using anti-PRMT5 antibody (A00635-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRMT5 antigen affinity purified polyclonal antibody (Catalog # A00635-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRMT5 at approximately 72KD. The expected band size for PRMT5 is at 72KD.
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