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Immunohistochemical staining of human kidney shows strong nuclear positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong nuclear positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong nuclear positivity in cells in tubules.

Anti-PRMT6 Antibody

HPA059424
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPRMT6
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PRMT6
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55170
  • Target name
    PRMT6
  • Target description
    protein arginine methyltransferase 6
  • Target synonyms
    heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6; histone-arginine N-methyltransferase PRMT6; HMT1 hnRNP methyltransferase-like 6; HRMT1L6; protein arginine N-methyltransferase 6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96LA8
  • Protein Name
    Protein arginine N-methyltransferase 6
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of PRMT6 using anti-PRMT6 antibody (A02924-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRMT6 antigen affinity purified polyclonal antibody (Catalog # A02924-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRMT6 at approximately 40 kDa. The expected band size for PRMT6 is at 40 kDa.
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