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Immunohistochemical staining of human fallopian tube shows moderate positivity in plasma in blood vessels.
Immunohistochemical staining of human fallopian tube shows moderate positivity in plasma in blood vessels.
Immunohistochemical staining of human fallopian tube shows moderate positivity in plasma in blood vessels.

Anti-PROC Antibody

HPA005550
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetPROC
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PROC
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5624
  • Target name
    PROC
  • Target description
    protein C, inactivator of coagulation factors Va and VIIIa
  • Target synonyms
    activated protein C; anticoagulant protein C; APC; autoprothrombin IIA; blood coagulation factor XIV; PC; prepro-protein C; PROC1; Protein C-Nagoya; THPH3; THPH4; type I protein C; vitamin K-dependent protein C
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP04070
  • Protein Name
    Vitamin K-dependent protein C
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Protein C using anti-Protein C antibody (A01742). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Protein C antigen affinity purified polyclonal antibody (Catalog # A01742) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Protein C at approximately 52KD. The expected band size for Protein C is at 52KD.
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