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ProductsBack/Anti-Prolactin [2C10D5H11]

Anti-Prolactin [2C10D5H11]

AB03403-23.0-BT
Absolute Antibody
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPRL
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Prolactin [2C10D5H11]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The antibody specifically detected PRL by Western blot analysis. The antibody 2C10D5H11 and a commercial rabbit polyclonal antibody were used to stain pituitary tumor. Results showed the antibody specificity is comparable to the commercial antibody. Further, the sensitivity of the antibody 2C10D5H11 was higher than the commercial antibody. The antibody and a commercially available rabbit polyclonal antibody were simultaneously detected on the normal tissue chip, indicating that the specificity of the antibody in normal tissue was equivalent to that of the commercially available antibody (CN113956356A).
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2C10D5H11
  • Gene ID5617
  • Target name
    PRL
  • Target description
    prolactin
  • Target synonyms
    GHA1, prolactin, decidual prolactin, growth hormone A1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP01236
  • Protein Name
    Prolactin
  • Scientific Description
    This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Prolactin/PRL using anti-Prolactin/PRL antibody (A00601-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Prolactin/PRL antigen affinity purified polyclonal antibody (Catalog # A00601-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Prolactin/PRL at approximately 26 kDa. The expected band size for Prolactin/PRL is at 26 kDa.
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