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Immunohistochemical staining of human placenta shows strong membranous and cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows strong membranous and cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows strong membranous and cytoplasmic positivity in trophoblastic cells.

Anti-PROX2 Antibody

HPA045057
Atlas Antibodies
ApplicationsChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPROX2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PROX2 Antibody
  • Delivery Days Customer
    4
  • Applications
    ChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID283571
  • Target name
    PROX2
  • Target description
    prospero homeobox 2
  • Target synonyms
    PROX-2, prospero homeobox protein 2, homeobox prospero-like protein PROX2
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PROX2 using anti-PROX2 antibody (A14649-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PROX2 antigen affinity purified polyclonal antibody (Catalog # A14649-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PROX2 at approximately 66 kDa. The expected band size for PROX2 is at 66 kDa.
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