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Anti-PrPSc [6H10]

Research Use Only
Ab01143-23.0
Absolute Antibody
ApplicationsImmunoPrecipitation, ELISA, Neutralisation/Blocking
Product group Antibodies
ReactivityBovine, Mouse, Sheep
TargetPRNP
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-PrPSc [6H10]
  • Delivery Days Customer
    7
  • Antibody Specificity
    This antibody is specific for PrPSc, and binds to a conformational epitope that incudes the extreme C-terminus of PrP. It does not react with recombinant mouse PrP or denatured PrPSc.
  • Application Supplier Note
    The binding specificity of this antibody for PrPSc has been confirmed in ELISA analysis; this antibody reacts with non-denatured PrPSc, but not with recombinant mouse PrP or denatured PrPSc treated with more than 3 M GdnHCl (Horiuchi et al, 2009). This antibody has been shown to immunoprecipitate PrPSc from the brains of mice, sheep, and cattle infected with prions (Horiuchi et al, 2009). Pretreatment of purified PrPSc with this antibody results in a 1 log reduction in infectious titre (Horiuchi et al, 2009).This antibody has also been used to identify C-terminal conformational differences between prion strains (Saijo et al, 2016).As this antibody recognises a conformational epitope on PrPSc, it does not react with PrPSc in immunoblot analysis (Horiuchi et al, 2009).
  • Applications
    ImmunoPrecipitation, ELISA, Neutralisation/Blocking
  • Applications Supplier
    ELISA; IP; neutralising
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    6H10
  • Gene ID493887
  • Target name
    PRNP
  • Target description
    prion protein
  • Target synonyms
    major prion protein; prion; prion protein (p27-30) (Creutzfeldt-Jakob disease, Gerstmann-Strausler-Scheinker syndrome, fatal familial insomnia); Prp; PRPC; SIP
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP23907
  • Protein Name
    Major prion protein
  • Scientific Description
    This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity
    Bovine, Mouse, Sheep
  • Reactivity Supplier
    Mouse, sheep, cow
  • Reactivity Supplier Note
    This antibody was raised by immunising PrP-ablated (Prnp-/-) mice with PrPSc purified from the brains of mice infected with the Obihiro prion strain.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203