Anti-PrPSc [6H10]

Absolute Antibody

Anti-PrPSc [6H10]

7

This antibody is specific for PrPSc, and binds to a conformational epitope that incudes the extreme C-terminus of PrP. It does not react with recombinant mouse PrP or denatured PrPSc.

The binding specificity of this antibody for PrPSc has been confirmed in ELISA analysis; this antibody reacts with non-denatured PrPSc, but not with recombinant mouse PrP or denatured PrPSc treated with more than 3 M GdnHCl (Horiuchi et al, 2009). This antibody has been shown to immunoprecipitate PrPSc from the brains of mice, sheep, and cattle infected with prions (Horiuchi et al, 2009). Pretreatment of purified PrPSc with this antibody results in a 1 log reduction in infectious titre (Horiuchi et al, 2009).This antibody has also been used to identify C-terminal conformational differences between prion strains (Saijo et al, 2016).As this antibody recognises a conformational epitope on PrPSc, it does not react with PrPSc in immunoblot analysis (Horiuchi et al, 2009).

ImmunoPrecipitation, ELISA, Neutralisation/Blocking

ELISA; IP; neutralising

Research Use Only

Monoclonal

6H10

PRNP

prion protein

major prion protein; prion; prion protein (p27-30) (Creutzfeldt-Jakob disease, Gerstmann-Strausler-Scheinker syndrome, fatal familial insomnia); Prp; PRPC; SIP

Rabbit

IgG

Major prion protein

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Bovine, Mouse, Sheep

Mouse, sheep, cow

This antibody was raised by immunising PrP-ablated (Prnp-/-) mice with PrPSc purified from the brains of mice infected with the Obihiro prion strain.

-20°C,2°C to 8°C

12352203