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Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuropil.
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuropil.
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuropil.

Anti-PRSS35 Antibody

HPA038788
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPRSS35
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PRSS35 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID167681
  • Target name
    PRSS35
  • Target description
    serine protease 35
  • Target synonyms
    C6orf158, dJ223E3.1, inactive serine protease 35, protease, serine 35
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N3Z0
  • Protein Name
    Inactive serine protease 35
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of PRSS35 using anti-PRSS35 antibody (A14920-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRSS35 antigen affinity purified polyclonal antibody (Catalog # A14920-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRSS35 at approximately 53 kDa. The expected band size for PRSS35 is at 47 kDa.
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