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Immunohistochemical staining of human kidney shows no positivity in cells in tubules and cells in glomeruli as expected.
Immunohistochemical staining of human kidney shows no positivity in cells in tubules and cells in glomeruli as expected.
Immunohistochemical staining of human kidney shows no positivity in cells in tubules and cells in glomeruli as expected.

Anti-PSD3 Antibody

HPA002455
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPSD3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PSD3 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23362
  • Target name
    PSD3
  • Target description
    pleckstrin and Sec7 domain containing 3
  • Target synonyms
    EFA6D, EFA6R, HCA67, PH and SEC7 domain-containing protein 3, ADP-ribosylation factor guanine nucleotide factor 6, epididymis tissue protein Li 20mP, exchange factor for ADP-ribosylation factor guanine nucleotide factor 6 D, exchange factor for ARF6 D, hepatocellular carcinoma-associated antigen 67, pleckstrin homology and SEC7 domain-containing protein 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NYI0
  • Protein Name
    PH and SEC7 domain-containing protein 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of PSD3 using anti-PSD3 antibody (A10329-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSD3 antigen affinity purified polyclonal antibody (Catalog # A10329-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSD3 at approximately 130 kDa. The expected band size for PSD3 is at 116 kDa.
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