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Anti-PSMC1 Antibody

144-61161
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPSMC1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-PSMC1 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5700
  • Target name
    PSMC1
  • Target description
    proteasome 26S subunit, ATPase 1
  • Target synonyms
    NEDGTH, P26S4, RPT2, S4, p56, 26S proteasome regulatory subunit 4, 26S protease regulatory subunit 4, 26S proteasome AAA-ATPase subunit RPT2, proteasome (prosome, macropain) 26S subunit, ATPase, 1, proteasome 26S ATPase subunit 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP62191
  • Protein Name
    26S proteasome regulatory subunit 4
  • Scientific Description
    PSMC1 Polyclonal Antibody
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PSMC1 using anti-PSMC1 antibody (A05555-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMC1 antigen affinity purified polyclonal antibody (Catalog # A05555-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMC1 at approximately 57 kDa. The expected band size for PSMC1 is at 49 kDa.
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