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Anti-PSMD6 (Center) Antibody

102-23826
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetPSMD6
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-PSMD6 (Center) Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    RB42752
  • Gene ID9861
  • Target name
    PSMD6
  • Target description
    proteasome 26S subunit, non-ATPase 6
  • Target synonyms
    Rpn7, S10, SGA-113M, p42A, p44S10, 26S proteasome non-ATPase regulatory subunit 6, breast cancer-associated protein SGA-113M, phosphonoformate immuno-associated protein 4, proteasome (prosome, macropain) 26S subunit, non-ATPase, 6, proteasome regulatory particle subunit p44S10
  • Host
    Rabbit
  • Protein IDQ15008
  • Protein Name
    26S proteasome non-ATPase regulatory subunit 6
  • Scientific Description
    Rabbit Anti-PSMD6 (Center) Antibody, 400 microl
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PSMD6 using anti-PSMD6 antibody (A09338-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMD6 antigen affinity purified polyclonal antibody (Catalog # A09338-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMD6 at approximately 46 kDa. The expected band size for PSMD6 is at 46 kDa.
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