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Immunohistochemical staining of human testis shows moderate nuclear and cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate nuclear and cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate nuclear and cytoplasmic positivity in cells in seminiferous ducts.

Anti-PSMD6 Antibody

HPA036922
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetPSMD6
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PSMD6
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9861
  • Target name
    PSMD6
  • Target description
    proteasome 26S subunit, non-ATPase 6
  • Target synonyms
    26S proteasome non-ATPase regulatory subunit 6; breast cancer-associated protein SGA-113M; p42A; p44S10; phosphonoformate immuno-associated protein 4; proteasome (prosome, macropain) 26S subunit, non-ATPase, 6; proteasome regulatory particle subunit p44S10; Rpn7; S10; SGA-113M
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15008
  • Protein Name
    26S proteasome non-ATPase regulatory subunit 6
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PSMD6 using anti-PSMD6 antibody (A09338-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMD6 antigen affinity purified polyclonal antibody (Catalog # A09338-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMD6 at approximately 46 kDa. The expected band size for PSMD6 is at 46 kDa.
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