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Immunohistochemical staining of human lung shows weak granular cytoplasmic positivity in macrophages.
Immunohistochemical staining of human lung shows weak granular cytoplasmic positivity in macrophages.
Immunohistochemical staining of human lung shows weak granular cytoplasmic positivity in macrophages.

Anti-PTGIS Antibody

HPA014193
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPTGIS
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PTGIS Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5740
  • Target name
    PTGIS
  • Target description
    prostaglandin I2 synthase
  • Target synonyms
    CYP8, CYP8A1, PGIS, PTGI, prostacyclin synthase, cytochrome P450, family 8, subfamily A, polypeptide 1, hydroperoxy icosatetraenoate dehydratase, prostaglandin I2 (prostacyclin) synthase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16647
  • Protein Name
    Prostacyclin synthase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of PTGIS/PGIS using anti-PTGIS/PGIS antibody (A05053-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human SK-OV-3 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human SiHa whole cell lysates, Lane 5: rat ovary tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse ovary tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGIS/PGIS antigen affinity purified polyclonal antibody (Catalog # A05053-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTGIS/PGIS at approximately 57 kDa. The expected band size for PTGIS/PGIS is at 57 kDa.
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