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Immunohistochemical staining of human Lymph node shows moderate cytoplasmic positivity in subset of non-germinal center cells.
Immunohistochemical staining of human Lymph node shows moderate cytoplasmic positivity in subset of non-germinal center cells.
Immunohistochemical staining of human Lymph node shows moderate cytoplasmic positivity in subset of non-germinal center cells.

Anti-PTPN22 Antibody

HPA004912
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPTPN22
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PTPN22
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID26191
  • Target name
    PTPN22
  • Target description
    protein tyrosine phosphatase non-receptor type 22
  • Target synonyms
    LYP, LYP1, LYP2, PEP, PTPN22.5, PTPN22.6, PTPN8, tyrosine-protein phosphatase non-receptor type 22, PEST-domain phosphatase, hematopoietic cell protein-tyrosine phosphatase 70Z-PEP, lymphoid-specific protein tyrosine phosphatase, protein tyrosine phosphatase, non-receptor type 22 (lymphoid), protein tyrosine phosphatase, non-receptor type 8
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of PTPN22 using anti-PTPN22 antibody (A00581-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: rat lung tissue lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse lung tissue lysates, Lane 6: mouse Neuro-2a whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTPN22 antigen affinity purified polyclonal antibody (Catalog # A00581-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTPN22 at approximately 100KD. The expected band size for PTPN22 is at 92KD.
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