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Immunohistochemical staining of human endometrium shows moderate to strong nuclear positivity in glandular cells.
Immunohistochemical staining of human endometrium shows moderate to strong nuclear positivity in glandular cells.
Immunohistochemical staining of human endometrium shows moderate to strong nuclear positivity in glandular cells.

Anti-PUS7 Antibody

HPA024116
Atlas Antibodies
ApplicationsWestern Blot
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPUS7
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PUS7
  • Delivery Days Customer
    4
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54517
  • Target name
    PUS7
  • Target description
    pseudouridine synthase 7
  • Target synonyms
    IDDABS; pseudouridylate synthase 7 (putative); pseudouridylate synthase 7 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96PZ0
  • Protein Name
    Pseudouridylate synthase 7 homolog
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PUS7 using anti-PUS7 antibody (A16132-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PUS7 antigen affinity purified polyclonal antibody (Catalog # A16132-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PUS7 at approximately 75 kDa. The expected band size for PUS7 is at 75 kDa.
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