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Immunohistochemical staining of human colon shows weak cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows weak cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows weak cytoplasmic positivity in glandular cells.

Anti-PUS7L Antibody

HPA058750
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPUS7L
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PUS7L Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID83448
  • Target name
    PUS7L
  • Target description
    pseudouridine synthase 7 like
  • Target synonyms
    pseudouridylate synthase PUS7L, pseudouridylate synthase 7 homolog-like protein, pseudouridylate synthase 7 like
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H0K6
  • Protein Name
    Pseudouridylate synthase PUS7L
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of PUS7L using anti-PUS7L antibody (A14523-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PUS7L antigen affinity purified polyclonal antibody (Catalog # A14523-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PUS7L at approximately 85 kDa. The expected band size for PUS7L is at 85 kDa.
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