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Immunohistochemical staining of human testis shows moderate granular cytoplasmic positivity in Leydig cells.
Immunohistochemical staining of human testis shows moderate granular cytoplasmic positivity in Leydig cells.
Immunohistochemical staining of human testis shows moderate granular cytoplasmic positivity in Leydig cells.

Anti-PYCR2 Antibody

HPA056873
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPYCR2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PYCR2 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID29920
  • Target name
    PYCR2
  • Target description
    pyrroline-5-carboxylate reductase 2
  • Target synonyms
    HLD10, P5CR2, pyrroline-5-carboxylate reductase 2, P5C reductase 2, pyrroline-5-carboxylate reductase family member 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96C36
  • Protein Name
    Pyrroline-5-carboxylate reductase 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of PYCR2 using anti-PYCR2 antibody (A10327-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat thymus tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PYCR2 antigen affinity purified polyclonal antibody (Catalog # A10327-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PYCR2 at approximately 35 kDa. The expected band size for PYCR2 is at 34,35 kDa.
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