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Immunohistochemical staining of human placenta shows cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows cytoplasmic positivity in trophoblastic cells.

Anti-RAB5A Antibody

HPA049354
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetRAB5A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RAB5A
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5868
  • Target name
    RAB5A
  • Target description
    RAB5A, member RAS oncogene family
  • Target synonyms
    RAB5; RAS-associated protein RAB5A; ras-related protein Rab-5A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP20339
  • Protein Name
    Ras-related protein Rab-5A
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RAB5A/B/C using anti-RAB5A/B/C antibody (A01891). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB5A/B/C antigen affinity purified polyclonal antibody (Catalog # A01891) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB5A/B/C at approximately 24 kDa. The expected band size for RAB5A/B/C is at 24 kDa.
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