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Immunohistochemical staining of human testis shows strong positivity in nuclear membrane in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong positivity in nuclear membrane in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong positivity in nuclear membrane in cells in seminiferous ducts.

Anti-RANBP2 Antibody

HPA018437
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRANBP2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RANBP2
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5903
  • Target name
    RANBP2
  • Target description
    RAN binding protein 2
  • Target synonyms
    ADANE, ANE1, IIAE3, NUP358, TRP1, TRP2, E3 SUMO-protein ligase RanBP2, 358 kDa nucleoporin, E3 SUMO-protein transferase RanBP2, P270, acute necrotizing encephalopathy 1 (autosomal dominant), nuclear pore complex protein Nup358, nucleoporin 358, nucleoporin Nup358, ran-binding protein 2, transformation-related protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49792
  • Protein Name
    E3 SUMO-protein ligase RanBP2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of RANBP2 using anti-RANBP2 antibody (A00981-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RANBP2 antigen affinity purified polyclonal antibody (Catalog # A00981-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RANBP2 at approximately 358 kDa. The expected band size for RANBP2 is at 358 kDa.
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