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Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil and neurons.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil and neurons.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil and neurons.

Anti-RAP1GDS1 Antibody

HPA019060
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRAP1GDS1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RAP1GDS1 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5910
  • Target name
    RAP1GDS1
  • Target description
    Rap1 GTPase-GDP dissociation stimulator 1
  • Target synonyms
    GDS1, SmgGDS, rap1 GTPase-GDP dissociation stimulator 1, RAP1, GTP-GDP dissociation stimulator 1, SMG GDS protein, SMG P21 stimulatory GDP/GTP exchange protein, exchange factor smgGDS
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP52306
  • Protein Name
    Rap1 GTPase-GDP dissociation stimulator 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of RAP1GDS1 using anti-RAP1GDS1 antibody (A07861-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAP1GDS1 antigen affinity purified polyclonal antibody (Catalog # A07861-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAP1GDS1 at approximately 66 kDa. The expected band size for RAP1GDS1 is at 66 kDa.
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