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Immunofluorescent staining of human cell line HEL shows localization to plasma membrane & cytosol.
Immunofluorescent staining of human cell line HEL shows localization to plasma membrane & cytosol.
Immunofluorescent staining of human cell line HEL shows localization to plasma membrane & cytosol.

Anti-RAPGEF2 Antibody

HPA068798
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetRAPGEF2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RAPGEF2 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9693
  • Target name
    RAPGEF2
  • Target description
    Rap guanine nucleotide exchange factor 2
  • Target synonyms
    CNrasGEF, NRAPGEP, PDZ-GEF1, PDZGEF1, RA-GEF, RA-GEF-1, RAGEF, Rap-GEP, nRap GEP, rap guanine nucleotide exchange factor 2, PDZ domain containing guanine nucleotide exchange factor (GEF) 1, PDZ domain-containing guanine nucleotide exchange factor 1, RA(Ras/Rap1A-associating)-GEF, Rap guanine nucleotide exchange factor (GEF) 2, cyclic nucleotide ras GEF, neural RAP guanine nucleotide exchange protein, ras/Rap1-associating GEF-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y4G8
  • Protein Name
    Rap guanine nucleotide exchange factor 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of RAPGEF2 using anti-RAPGEF2 antibody (A04621-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAPGEF2 antigen affinity purified polyclonal antibody (Catalog # A04621-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAPGEF2 at approximately 200-220KD. The expected band size for RAPGEF2 is at 200-220KD.
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Immunofluorescence staining of Hela cells with CSB-PA897493LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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