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Immunohistochemical staining of human spleen shows distinct nuclear positivity in cells of the spleen.
Immunohistochemical staining of human spleen shows distinct nuclear positivity in cells of the spleen.
Immunohistochemical staining of human spleen shows distinct nuclear positivity in cells of the spleen.

Anti-RAPGEF5 Antibody

HPA057821
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRAPGEF5
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RAPGEF5 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9771
  • Target name
    RAPGEF5
  • Target description
    Rap guanine nucleotide exchange factor 5
  • Target synonyms
    GFR, MR-GEF, MRGEF, REPAC, rap guanine nucleotide exchange factor 5, M-Ras-regulated GEF, M-Ras-regulated Rap GEF, Rap guanine nucleotide exchange factor (GEF) 5, guanine nucleotide exchange factor for Rap1, related to Epac
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92565
  • Protein Name
    Rap guanine nucleotide exchange factor 5
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of RAPGEF5 using anti-RAPGEF5 antibody (A12923-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Hacat whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAPGEF5 antigen affinity purified polyclonal antibody (Catalog # A12923-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAPGEF5 at approximately 75 kDa. The expected band size for RAPGEF5 is at 68 kDa.
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