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Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.

Anti-RASAL2 Antibody

HPA020453
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetRASAL2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RASAL2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9462
  • Target name
    RASAL2
  • Target description
    RAS protein activator like 2
  • Target synonyms
    NGAP; ras GTPase-activating protein nGAP; Ras protein activator like 1; RASAL2/ACVR1 fusion
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UJF2
  • Protein Name
    Ras GTPase-activating protein nGAP
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Lane 1: Human Hela cell lysates; Lane 2: Human MDA-MB-231 cell lysates; Lane 3: Human MKN45 cell lysates; Lane 4: Human HepG2 celll lysates probed with RASAL2 Polyclonal Antibody, Unconjugated (bs-21160R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: Mouse brain tissue, Primary antibody: CSB-PA151979(RASAL2 Antibody) at dilution 1/350, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds
Product group Antibodies
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RASAL2 AntibodyCSB-PA151979
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse
TargetRASAL2
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Atlas Antibodies
PrEST Antigen RASAL2APREST74618
Protein IDQ9UJF2
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Product group Antibodies
LifeSpan BioSciences
NGAP / RASAL2 AntibodyLS-C750092
ApplicationsWestern Blot
TargetRASAL2
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Figure 1. Western blot analysis of RASAL2 using anti-RASAL2 antibody (A07356-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RASAL2 antigen affinity purified polyclonal antibody (Catalog # A07356-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RASAL2 at approximately 150 kDa. The expected band size for RASAL2 is at 129 kDa.
Product group Antibodies
Boster Bio
Anti-RASAL2 Antibody Picoband(r)A07356-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
TargetRASAL2
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Product group Antibodies
References
GeneTex
RASAL2 antibody [C2C3], C-termGTX120989
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetRASAL2
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