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Immunohistochemical staining of human kidney shows strong membranous and cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong membranous and cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong membranous and cytoplasmic positivity in cells in tubules.

Anti-RASD1 Antibody

HPA047896
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetRASD1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RASD1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51655
  • Target name
    RASD1
  • Target description
    ras related dexamethasone induced 1
  • Target synonyms
    activator of G-protein signaling 1; AGS1; dexamethasone-induced Ras-related protein 1; DEXRAS1; MGC:26290; RAS, dexamethasone-induced 1; ras-related protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y272
  • Protein Name
    Dexamethasone-induced Ras-related protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Dexras1/RASD1 using anti-Dexras1/RASD1 antibody (A05991-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat RH35 whole cell lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dexras1/RASD1 antigen affinity purified polyclonal antibody (Catalog # A05991-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dexras1/RASD1 at approximately 32 kDa. The expected band size for Dexras1/RASD1 is at 32 kDa.
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