
Figure 1. Western blot analysis of RBBP8 using anti-RBBP8 antibody (A02076-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBBP8 antigen affinity purified polyclonal antibody (Catalog # A02076-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RBBP8 at approximately 130 kDa. The expected band size for RBBP8 is at 102 kDa.
Anti-RBBP8 Antibody Picoband(r)
A02076-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetRBBP8
Overview
- SupplierBoster Bio
- Product NameAnti-RBBP8 Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID5932
- Target nameRBBP8
- Target descriptionRB binding protein 8, endonuclease
- Target synonymsCOM1; CTBP-interacting protein; CTIP; DNA endonuclease RBBP8; JWDS; RBBP-8; retinoblastoma binding protein 8; RIM; SAE2; SCKL2; sporulation in the absence of SPO11 protein 2 homolog
- HostRabbit
- IsotypeIgG
- Protein IDQ99708
- Protein NameDNA endonuclease RBBP8
- Scientific DescriptionBoster Bio Anti-RBBP8 Antibody Picoband® catalog # A02076-2. Tested in WB, IHC, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203