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Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in endocrine cells.
Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in endocrine cells.
Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in endocrine cells.

Anti-RBM15B Antibody

HPA036645
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRBM15B
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RBM15B Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID29890
  • Target name
    RBM15B
  • Target description
    RNA binding motif protein 15B
  • Target synonyms
    HUMAGCGB, HsOTT3, OTT3, putative RNA-binding protein 15B, chromosome 3p21.1 gene sequence, huOTT3, one twenty two protein 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8NDT2
  • Protein Name
    Putative RNA-binding protein 15B
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of RBM15B using anti-RBM15B antibody (A11484-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBM15B antigen affinity purified polyclonal antibody (Catalog # A11484-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RBM15B at approximately 110 kDa. The expected band size for RBM15B is at 97 kDa.
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