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Immunofluorescent staining of human cell line HaCaT shows localization to nuclear speckles.
Immunofluorescent staining of human cell line HaCaT shows localization to nuclear speckles.
Immunofluorescent staining of human cell line HaCaT shows localization to nuclear speckles.

Anti-RBM25 Antibody

HPA070713
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetRBM25
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RBM25
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID58517
  • Target name
    RBM25
  • Target description
    RNA binding motif protein 25
  • Target synonyms
    NET52, RED120, RNPC7, S164, Snu71, fSAP94, RNA-binding protein 25, Arg/Glu/Asp-rich protein, 120 kDa, RNA-binding region (RNP1, RRM) containing 7, RNA-binding region-containing protein 7, U1 small nuclear ribonucleoprotein 1SNRP homolog, arg/Glu/Asp-rich protein of 120 kDa, functional spliceosome-associated protein 94
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49756
  • Protein Name
    RNA-binding protein 25
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of RBM25 using anti-RBM25 antibody (A07494-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBM25 antigen affinity purified polyclonal antibody (Catalog # A07494-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RBM25 at approximately 120 kDa. The expected band size for RBM25 is at 100 kDa.
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