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anti-REXO4 / PMC2 antibody

ARG40150
Arigo Biolaboratories
ApplicationsWestern Blot
Product group Antibodies
TargetREXO4
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Overview

  • Supplier
    Arigo Biolaboratories
  • Product Name
    anti-REXO4 / PMC2 antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 - 1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID57109
  • Target name
    REXO4
  • Target description
    REX4 homolog, 3'-5' exonuclease
  • Target synonyms
    exonuclease XPMC2; REX4; REX4, RNA exonuclease 4 homolog; RNA exonuclease 4; Xenopus prevents mitotic catastrophe 2 homolog; XPMC2; XPMC2 prevents mitotic catastrophe 2 homolog; XPMC2H
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of REXO4 using anti-REXO4 antibody (A13732-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-REXO4 antigen affinity purified polyclonal antibody (Catalog # A13732-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for REXO4 at approximately 50 kDa. The expected band size for REXO4 is at 47 kDa.
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