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Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in decidual cells.
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in decidual cells.
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in decidual cells.

Anti-RGL4 Antibody

HPA035979
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetRGL4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RGL4
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID266747
  • Target name
    RGL4
  • Target description
    ral guanine nucleotide dissociation stimulator like 4
  • Target synonyms
    RalGDS related oncogene; Ral-GDS related protein Rgr; ralGDS-like 4; ral-GDS-related protein; Rgr
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IZJ4
  • Protein Name
    Ral-GDS-related protein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RGL4 using anti-RGL4 antibody (A16655-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RGL4 antigen affinity purified polyclonal antibody (Catalog # A16655-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RGL4 at approximately 52 kDa. The expected band size for RGL4 is at 52 kDa.
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