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Immunohistochemical staining of human small intestine shows cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human small intestine shows cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human small intestine shows cytoplasmic positivity in glandular cells.

Anti-RHOT2 Antibody

HPA012895
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
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Product group Antibodies
TargetRHOT2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-RHOT2
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID89941
  • Target name
    RHOT2
  • Target description
    ras homolog family member T2
  • Target synonyms
    ARHT2; C16orf39; MIRO2; MIRO-2; mitochondrial Rho (MIRO) GTPase 2; mitochondrial Rho GTPase 2; ras homolog gene family, member T2; RASL
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IXI1
  • Protein Name
    Mitochondrial Rho GTPase 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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IHC image of CSB-PA810289LA01HU diluted at 1:400 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of MIRO2/RHOT2 using anti-MIRO2/RHOT2 antibody (A08801-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIRO2/RHOT2 antigen affinity purified polyclonal antibody (A08801-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MIRO2/RHOT2 at approximately 80 kDa. The expected band size for MIRO2/RHOT2 is at 68 kDa.
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