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ProductsBack/Anti-Ribonuclease A/RNASE1 Antibody Picoband(r)
Anti-Ribonuclease A antibody, PA1507, Western blotting All lanes: Anti Ribonuclease A (PA1507) at 0.5ug/ml Lane 1: MCF-7 Whole Cell Lysate at 40ug Lane 2: U87 Whole Cell Lysate at 40ug Lane 3: HELA Whole Cell Lysate at 40ug Lane 4: MM231 Whole Cell Lysate at 40ug Lane 5: JURKAT Whole Cell Lysate at 40ug Lane 6: SMMC Whole Cell Lysate at 40ug Lane 7: COLO320 Whole Cell Lysate at 40ug Lane 8: SW620 Whole Cell Lysate at 40ug Lane 9: CEM Whole Cell Lysate at 40ug Predicted bind size: 18KD Observed bind size: 18KD
Anti-Ribonuclease A antibody, PA1507, Western blotting All lanes: Anti Ribonuclease A (PA1507) at 0.5ug/ml Lane 1: MCF-7 Whole Cell Lysate at 40ug Lane 2: U87 Whole Cell Lysate at 40ug Lane 3: HELA Whole Cell Lysate at 40ug Lane 4: MM231 Whole Cell Lysate at 40ug Lane 5: JURKAT Whole Cell Lysate at 40ug Lane 6: SMMC Whole Cell Lysate at 40ug Lane 7: COLO320 Whole Cell Lysate at 40ug Lane 8: SW620 Whole Cell Lysate at 40ug Lane 9: CEM Whole Cell Lysate at 40ug Predicted bind size: 18KD Observed bind size: 18KD
Anti-Ribonuclease A antibody, PA1507, Western blotting All lanes: Anti Ribonuclease A (PA1507) at 0.5ug/ml Lane 1: MCF-7 Whole Cell Lysate at 40ug Lane 2: U87 Whole Cell Lysate at 40ug Lane 3: HELA Whole Cell Lysate at 40ug Lane 4: MM231 Whole Cell Lysate at 40ug Lane 5: JURKAT Whole Cell Lysate at 40ug Lane 6: SMMC Whole Cell Lysate at 40ug Lane 7: COLO320 Whole Cell Lysate at 40ug Lane 8: SW620 Whole Cell Lysate at 40ug Lane 9: CEM Whole Cell Lysate at 40ug Predicted bind size: 18KD Observed bind size: 18KD

Anti-Ribonuclease A/RNASE1 Antibody Picoband(r)

PA1507
Boster Bio
ApplicationsWestern Blot
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetRNASE1
100 ug
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-Ribonuclease A Antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
  • Applications
    Western Blot
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Gene ID6035
  • Target name
    RNASE1
  • Target description
    ribonuclease A family member 1, pancreatic
  • Target synonyms
    HP-RNase; RAC1; RIB1; RIB-1; ribonuclease 1; ribonuclease A C1; ribonuclease pancreatic; ribonuclease, RNase A family, 1 (pancreatic); RNase 1; RNase A; RNase upI-1; RNS1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP07998
  • Protein Name
    Ribonuclease pancreatic
  • Scientific Description
    Boster Bio Anti-Ribonuclease A/RNASE1 Antibody catalog # PA1507. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RNASE1 using anti-RNASE1 antibody (A03553-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat pancreas tissue lysates, Lane 2: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNASE1 antigen affinity purified polyclonal antibody (Catalog # A03553-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNASE1 at approximately 18 kDa. The expected band size for RNASE1 is at 18 kDa.
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